FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_mr75510-1.352000000a2c00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_mr75510-1.352000000a2c00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9205939
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA290110.31513352413045537No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA222930.24215889329703358No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA202800.22029257417412826No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG184200.2000882256551993No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG182570.19831762952155124No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG177710.19303842877950853No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC165280.17953627544132109No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA163160.17723341421228186No Hit
CTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAG160080.1738877478983947No Hit
CTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATA157480.17106348412693154No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT133380.14488473147606126No Hit
GGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC130100.1413218141028308No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT122050.1325774589642621No Hit
CTCGGGAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACAT120410.13079600027764685No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA116240.12626631569033858No Hit
CAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACA115720.12570146293604598No Hit
GGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTT114270.12412639275580688No Hit
CAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTGTG112000.12166059323226017No Hit
CCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCATC111350.12095452728939438No Hit
GGCTCATGTGGCTTTCGGAAATTGAACCAGCCAGAATTGCATGTGTCCTC109130.11854304053068351No Hit
GTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGGCAACGACTGT108300.11764144863440872No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT107970.11728298438649223No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA107680.11696797035044443No Hit
GGTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGAC107480.1167507192911011No Hit
GCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTTC107190.11643570525505328No Hit
GTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAGGACGC107140.11638139249021745No Hit
GGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAAA106160.11531686229943519No Hit
TGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTCC105610.11471942188624104No Hit
GGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCC104470.1134810908479841No Hit
GCGGAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGG102620.11147151854905839No Hit
GGCAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTG101360.11010283687519545No Hit
GGCGGCTACCTTATCGGCCTCAGGCCGATAGAGCGAGGCTATGAGGCCAT100700.1093859083793625No Hit
CCGGTGTCTGCCCCAGCATGCCAAGGCAATGGGATGTCATGAAACCACTC99420.10799550159956524No Hit
CTGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTC98150.10661595737273513No Hit
CCTCAATCCACACTCTATTCCACACCATGAGCATGTCCTCAGTGGTCATC97410.10581212845316484No Hit
GGAGCATCCGGGAGACTGCCTGTCTTGCAAAATCATATGCGCAGATGTGG96200.10449775954413776No Hit
GCGGGAGTGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCC95900.10417188295512277No Hit
CGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCA94970.10316166552917633No Hit
GGCCAGTGCATGGCTCATGTGGCTTTCGGAAATTGAACCAGCCAGAATTG93810.10190160938498506No Hit
GCTGGGAACAGTTTCACTGGGGATCTTCTTCGTCTTGATGCGGAATAAGG93610.10168435832564175No Hit
GGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCATG92920.10093484217090729No Hit
GTAAATCTCAAAAAGGCTAGTATCGCCAAAACCATTCTGATGGGTCCATG92290.10025050133397582No Hit
GGGCCCACCTGATTGAGATGAAAACATGTGAATGGCCAAAGTCTCACACA92270.1002287762280415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC3400.091.691181
GCCCGGT3000.079.751
CGCTAAT2350.077.127661
GGCATAT2350.074.042561
ACCGTAC1100.072.500011
GTCCGTA2450.071.020411
CGTCCAT5350.069.112151
CGGTGCT7000.068.357151
CGCGGAT2050.067.195121
GCCGGCT2650.065.660381
GTGTCTA2050.063.6585354
GCCCGTA808.096322E-963.43751
TCTTATT8800.061.7897769
GGCCTCT7700.061.20131
GCGGGCT3200.061.1718751
CGGTCGT2850.061.0526351
GCCGCGT4450.060.2809031
GCGGGTA2650.060.1886751
CGGGGAG16900.059.2011831
GCGGGTT3300.057.1212121
Produced by FastQC (version 0.11.2)