FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_672.352000000a2d29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_672.352000000a2d29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC840.5206719147089817Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATA360.22314510630384926Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAGC170.10537407797681769Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG100.007026653145.01
GATCGGG151.2078222E-4145.01
TACGGGA100.007026653145.02
TACGGAG100.007026653145.03
TCGGAAA306.1118044E-10145.04
TCCAGGA100.007026653145.02
CTCGGAA100.007026653145.03
GAGAACG306.1118044E-10145.07
ACGGAGA151.2078222E-4145.04
GCGGGAG151.2078222E-4145.03
GGACCGG306.1118044E-10145.01
GTCCAGG100.007026653145.01
CGGAGAG500.0145.05
TCGAAGA100.007026653145.04
ATGGGAG850.0136.470583
CGGAAAG500.0130.55
CCGGAGA403.092282E-11126.8754
GATGGGA750.0125.666672
CGGAAGG351.7844286E-9124.285715
ACGGGAG700.0124.285713
Produced by FastQC (version 0.11.2)