FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_671.352000000a2d1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_671.352000000a2d1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8320
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC1311.5745192307692308Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATT200.2403846153846154Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATA160.19230769230769232Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGAGC130.15625Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGAAC120.14423076923076925Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGGAGATC90.10817307692307693Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAA151.1868604E-4145.000025
CCGGAAA100.0069655613145.04
GACGGGA359.094947E-12145.02
TTGGGAA202.0073167E-6145.03
AGAGCGG253.3935066E-8145.08
ACGGGAG253.3935066E-8145.03
GAGAGCG253.3935066E-8145.07
GCACCGG100.0069655613145.01
GAGGGCT100.0069655613145.09
GTAAAAG100.0069655613145.06
CGGGAGA351.6789272E-9124.285714
AAAGGGC301.0075382E-7120.833349
GAGGGGC301.0075382E-7120.833349
GAGCGGG203.7309364E-4108.759
ACGGGAA203.7309364E-4108.753
GGACGGG501.6552804E-10101.51
GGAGAGC301.507418E-596.666686
GTTGGGA301.507418E-596.666682
CGGGAAA553.5288394E-1092.272724
CCGGAGA259.05986E-487.04
Produced by FastQC (version 0.11.2)