FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_662.352000000a2d0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_662.352000000a2d0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15539
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC1120.7207671021301242Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG100.007024132145.01
GATCCGG151.2069544E-4145.01
TACGGAG100.007024132145.03
TACGGAA100.007024132145.03
TCTCGGG100.007024132145.0145
GATCTGG151.2069544E-4145.01
GTACGGA100.007024132145.02
CGGAGAG301.05086656E-7120.8333365
GGATGGG256.2646504E-6116.01
GATGGGA256.2646504E-6116.02
AAGGGGC256.2646504E-6116.09
GGAGAGG457.8216544E-11112.777786
ACGGAGA203.7938688E-4108.754
GAACGGG404.5074557E-9108.751
AACGGGA451.02190825E-896.6666642
ATCCGGA259.2121155E-487.02
GGAAAGC259.2121155E-487.06
ATCTGGA259.2121155E-487.02
GTCGGGA259.2121155E-487.02
ACGGGAA353.3314238E-582.857143
Produced by FastQC (version 0.11.2)