FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_661.352000000a2cf1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_661.352000000a2cf1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369915
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA20870.5641836638146601No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG9300.25140910749766837No Hit
CAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACA8430.227890190989822No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA6930.18734033494181096No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG6720.1816633550950894No Hit
CCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCAGCAGGAGGCCAA6250.16895773353337928No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA5960.1611180946974305No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA5690.1538191206087885No Hit
TGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGT5470.14787180838841354No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC5190.14030250192611815No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT5160.13949150480515796No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG4800.1297595393536353No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC4730.12786721273806145Illumina Single End PCR Primer 1 (97% over 35bp)
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA4700.12705621561710123No Hit
CTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAG4670.126245218496141No Hit
CTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATA4620.12489355662787396No Hit
TGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTCC4600.12435289188056715No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA4540.12273089763864672No Hit
GGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC4220.1140802616817377No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT4140.11191760269251044No Hit
CCTCAATCCACACTCTATTCCACACCATGAGCATGTCCTCAGTGGTCATC4110.11110660557155022No Hit
GGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCC4040.10921427895597637No Hit
GCTGTTTTGCGTTTTCCGGGGGGTCTCCTCTAACCACTAGTCCCTCTGCT4020.10867361420866956No Hit
AGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCA4000.10813294946136275No Hit
GTAAATCTCAAAAAGGCTAGTATCGCCAAAACCATTCTGATGGGTCCATG3980.10759228471405594No Hit
CCTGGACATTCCTCAAACCGGATTTCAAGCTCTTCACTGTGCCATGGCCC3930.1062406228457889No Hit
CAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTGTG3770.10191530486733438No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT3710.10029331062541395No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC100.0070890696145.01
TACGGGA100.0070890696145.02
CGGTCGT100.0070890696145.01
GACCGGA403.274181E-11126.8752
GAACCGG256.459448E-6116.01
GACGGAA203.864111E-4108.752
TTCGGAG203.864111E-4108.753
GCCCGGA203.864111E-4108.752
AAGGGGC1050.0103.571439
TCGGAGG301.598121E-596.6666644
GGACGGA301.598121E-596.6666641
GCCGGCT259.38204E-487.01
ACGGGAG950.083.947373
CCCCGGT451.2313449E-680.555551
GTTCGGA451.2313449E-680.555552
TCGGGAG502.3033645E-672.53
AAACGGC406.657643E-572.59
GCGGGAA406.657643E-572.53
CGGAGAA809.822543E-1172.55
GTCGGTT300.00193477572.51
Produced by FastQC (version 0.11.2)