FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_652.352000000a2ce4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_652.352000000a2ce4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17858
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC1200.6719677455482137Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATA380.21278978609026766Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGT100.0070330394145.000028
ACCGGAG100.0070330394145.000023
TATGGGG100.0070330394145.000028
GGACTGA100.0070330394145.000021
GGTCCGA100.0070330394145.000021
CCGGAAA100.0070330394145.000024
AATGCCT100.0070330394145.000028
CTTGAAC100.0070330394145.000026
GTCCGAA100.0070330394145.000022
TAAGGGC100.0070330394145.000029
GGATTGG100.0070330394145.000021
TTCGCAA100.0070330394145.000026
AGAGCGG202.0731986E-6145.000028
AATCGGA100.0070330394145.000022
TACCGGA100.0070330394145.000022
TGCAAGC100.0070330394145.000022
CGGAGAG450.0145.05
GGAGAGC256.291173E-6116.06
TGGAAAG256.291173E-6116.05
ACGGAGA256.291173E-6116.04
Produced by FastQC (version 0.11.2)