FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_651.352000000a2cd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_651.352000000a2cd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22474
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC900.40046275696360234Illumina Single End PCR Primer 1 (96% over 33bp)
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA330.14683634421998754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGC202.0850857E-6145.06
CTGCGAG100.0070450855145.07
TGCGAGA100.0070450855145.08
CGGAGTA202.0850857E-6145.0145
CGGAGAG351.0913936E-11145.05
CGGAGAA203.8164805E-4108.755
GTCAGAC353.364464E-582.857141
GACAGGA300.001911101472.52
CCGGAGA702.6502676E-972.54
GAGAGCG406.522442E-572.57
CAGACTG406.522442E-572.53
AGAGGGG651.3368481E-766.923088
TCAGACT451.1687131E-464.444442
GACTGCG451.1687131E-464.444445
CCCGGAG451.1687131E-464.444443
GAGAGGG702.2325548E-762.1428577
TCGGAAA350.003521270862.1428574
GAGAAGG350.003521270862.1428577
GGACAGG350.003521270862.1428571
AATCGGA350.003521270862.1428572
Produced by FastQC (version 0.11.2)