FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_642.352000000a2cca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_642.352000000a2cca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26425
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT100.007052084145.03
TAACAGC100.007052084145.0145
AGATTTA100.007052084145.04
GTGAGTC100.007052084145.01
GACTGCG100.007052084145.03
TGACTGC100.007052084145.02
GGAAAGG450.00953630448.3333326
CCAGCAG800.002000972636.255
AAAGGGG750.001304161729.0000028
GGGGGAA659.697833E-720.07692320-24
AAAAGGG456.8245427E-419.33333225-29
GGGGAAA955.7207217E-918.3157920-24
TGTTCAG3203.7739615E-415.859375145
GGGCGGG600.00467342414.510-14
GGGAAAA600.00467342414.520-24
GAGTGTT3455.8651884E-412.608696145
AAGAGTG4302.4974725E-98.430232145
AGAGTGT3603.8964572E-77.2499995140-144
AGGGAAA4452.751458E-76.516854135-139
GGGGGGG4903.407149E-86.510204315-19
Produced by FastQC (version 0.11.2)