FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_641.352000000a2cbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_641.352000000a2cbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17787
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC800.4497666835329173Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGG202.072873E-6144.999981
GTCACAA100.0070327097144.99998145
AGAGGCT100.0070327097144.999984
CCGCAAA100.0070327097144.999984
TAAGGGC259.2344906E-487.09
TTGGGAA353.3449258E-582.857143
TGGGAGA553.899806E-665.9090964
CGGAAGG350.003509077762.1428575
GAAAGCG350.003509077762.1428577
TTGGAAA606.5351196E-660.4166684
TGGAAAG501.9566945E-458.05
AAAGGGC400.005953816754.3749969
GTTGGGA400.005953816754.3749962
GAGGGGC400.005953816754.3749969
GAAGGGC400.005953816754.3749969
GGAGAGG901.25101E-648.3333366
AGAGGGG1109.239011E-846.1363688
GAGAGGG951.8157807E-645.7894747
ATTGGAA700.001028815341.428573
AAGAGGG800.00198329136.2499968
Produced by FastQC (version 0.11.2)