FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_631.352000000a2c94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_631.352000000a2c94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73823
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTACT400.006028789654.3756
CTTCTTG450.0096040748.3333325
TGTGCCC1402.7126445E-531.071428145
AACTCAC1000.00603856329.05
TCACCTG1850.00500355719.5945938
TCTCGGT1655.618242E-517.575758145
CTCGGTG1750.00988797716.571428145
TAGATCT2703.057521E-710.740741145
CCAGAAT1150.00274175210.086956105-109
GAGTGTG9302.928573E-109.354839145
CCTCTAT5503.4292316E-79.227272145
GTAGATC3306.29028E-68.787879145
GTGTAGA4007.2759576E-128.7135-139
AAAGAGT11000.08.568181145
GCCTCTA5954.2862666E-88.529411145
GTAGGGA14751.2302189E-78.355932145
TGTAGAT3554.469257E-98.169014135-139
GATCTCG3205.563379E-88.15625140-144
TCGGTGG1800.00141755198.055555140-144
ATCTCGG3257.230301E-88.030769140-144
Produced by FastQC (version 0.11.2)