FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_622.352000000a2c87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_622.352000000a2c87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14660
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC150.10231923601637108Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCC350.00349677862.1428579
CGTGTAG2004.4048135E-410.875145
AAGAGTG1751.0377946E-49.114285140-144
AGAGTGT1604.2919483E-49.0625140-144
TAAAAAA1450.00178068769.070-74
AGGGAAA1851.8987714E-48.621621135-139
CGTCGTG2300.00195293486.934782140-144
GGGAAAG2150.0078614366.744186135-139
GTGTAGG3051.16393756E-46.6557374130-134
AGCGTCG2450.0037946976.510204125-129
AGAGCGT2600.0070488836.134616140-144
Produced by FastQC (version 0.11.2)