FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_612.352000000a2c6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_612.352000000a2c6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35554
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCG860.2418855824942341No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAA860.2418855824942341No Hit
GAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTT750.21094672891939023No Hit
GCGGGTTAGAGGGTTCATAACACAAGGGTAGTATCCCACCAGCGCCTCCT730.20532148281487317No Hit
GCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGG660.18563312144906338No Hit
GCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAG660.18563312144906338No Hit
CTCGGCTTAGGTCCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAA610.1715700061877707No Hit
GTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATGA600.1687573831355122No Hit
CCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCA550.1546942678742195No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCAACTTAACCTCGCATGCAATCG520.1462563987174439No Hit
CTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGTC500.1406311526129268No Hit
CCTCGACCGATTAGTATTGGTCCGCTCCAACTATCACTAGCCTTCCACTT410.11531754514259998No Hit
CCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCAT400.11250492209034146No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCGACTTAACCTCGCATGCAATCG400.11250492209034146No Hit
CACGGTTTCAGGATCTATTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT390.10969229903808292No Hit
CCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGT370.10406705293356584No Hit
GTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAA360.1012544298813073No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACTT100.0070623257145.000028
TCGAAGG100.0070623257145.000027
CGAAGGA100.0070623257145.000028
ACGCCGT400.00600318154.3750042
GGTCATT450.009563426548.3333321
CGTTCTA604.8805724E-448.3333322
GCGTTCT657.2411547E-444.6153831
TCTAAGG800.002010353636.2500045
CTAAGGG850.00270673234.117656
TAAGGGC900.0035817232.222227
AGGGCGT900.0035817232.222229
AAGGGCG1152.1435916E-431.5217368
GTTCTAA950.00466687130.5263183
TCTCGGG950.00466687130.526318145
AGCCCAC200.00606120929.0135-139
GTCGATC1100.001886836419.7727261
GTGACCG400.00789116618.1250022
CACTTCC400.00789116618.1250027
GCATTCC400.00789116618.12585-89
TCGATCG1253.600044E-517.42
Produced by FastQC (version 0.11.2)