FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_611.352000000a2c51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_611.352000000a2c51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83005
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTT3140.3782904644298536No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAA2660.3204626227335703No Hit
GCGGGTTAGAGGGTTCATAACACAAGGGTAGTATCCCACCAGCGCCTCCT2300.27709174146135773No Hit
GCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAG2230.26865851454731643No Hit
CTCGGCTTAGGTCCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAA1930.23251611348713933No Hit
GGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCG1810.21805915306306847No Hit
GCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGG1670.20119269923498584No Hit
CCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCA1660.19998795253297996No Hit
CTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGTC1520.1831214987048973No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACA1490.1795072585988796No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCGACTTAACCTCGCATGCAATCG1450.17468827179085597No Hit
CCTCGACCGATTAGTATTGGTCCGCTCCAACTATCACTAGCCTTCCACTT1410.16986928498283235No Hit
CGTCCCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTG1370.16505029817480873No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCAACTTAACCTCGCATGCAATCG1360.16384555147280286No Hit
CAAACACTCAATATCAAACTACAGTAAAGCTCCATGGGGTCTTTCCGTCC1340.16143605806879102No Hit
GTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATGA1300.15661707126076743No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC1210.1457743509427143No Hit
CCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCAT1170.14095536413469067No Hit
CGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGC1130.13613637732666709No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACACCGAT1110.13372688392265525No Hit
GGCGTAACGATTTGGGCACTGTCTCAACGAGAGACTCGGTGAAATTTTAG1050.12649840371061982No Hit
CACGGTTTCAGGATCTATTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT1040.12529365700861395No Hit
GGCCTATTCACTGCGGCTGACCGAAGTCAGCACCCCTTCTCCCGAAGTTA990.11926992349858441No Hit
CCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGT990.11926992349858441No Hit
GTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAA990.11926992349858441No Hit
GGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAA940.1132461899885549No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACAC930.11204144328654901No Hit
GGGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTA910.1096319498825372No Hit
CCCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGC900.1084272031805313No Hit
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTATGACT890.10722245647852538No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG870.10481296307451358No Hit
GTACAAACACTCAATATCAAACTACAGTAAAGCTCCATGGGGTCTTTCCG870.10481296307451358No Hit
GTAGGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGA850.10240346967050178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG406.067876E-790.625013
GAGCGGT259.3562313E-487.09
GTCGTGG259.3562313E-487.0145
CGGAAAA502.287632E-672.55
CCGGAAA300.001929472772.54
ATTGGGA300.001929472772.52
CTCGACC502.287632E-672.52
ATCGGAG300.001929472772.53
GGTCCGG350.003554994562.1428571
GATTGGG606.7509445E-660.4166681
AGGGAGA701.682431E-551.7857134
CCGATTA752.530247E-548.3333367
GCGGGTT1203.5524863E-948.3333321
CGCACGG901.308832E-648.3333323
GATAGGG1203.5524863E-948.3333321
TAGAGGG1402.6557245E-1046.6071437
GGCGCAC951.8995324E-645.7894741
ACCGATT1002.7038113E-643.56
GCGCACG1002.7038113E-643.52
GAGGGGC1851.8189894E-1243.1081059
Produced by FastQC (version 0.11.2)