FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_592.352000000a2c44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_592.352000000a2c44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325445
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA19090.5865814500145954No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG8920.27408625113306395No Hit
CAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACA8040.24704635191814284No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA7010.21539737897340563No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA6530.20064834303799411No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG6290.1932738250702884No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA5530.16992118483922014No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC5150.158244864723686No Hit
TGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGT4810.14779763093610288No Hit
CTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATA4270.13120496550876493No Hit
GGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC4200.12905406443485073No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG4150.12751770652491204No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT4110.1262886201969611No Hit
TGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTCC4020.12352317595907142No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA3900.11983591697521853No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA3890.1195286453932308No Hit
CTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAG3880.11922137381124306No Hit
CCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCAGCAGGAGGCCAA3850.11829955906527984No Hit
GGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCC3820.11737774431931662No Hit
AGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCA3780.11614865799136566No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT3730.11461230008142696No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT3660.1124613990075128No Hit
GCTGTTTTGCGTTTTCCGGGGGGTCTCCTCTAACCACTAGTCCCTCTGCT3470.10662323894974574No Hit
CAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTGTG3410.10477960945781929No Hit
CCTCAATCCACACTCTATTCCACACCATGAGCATGTCCTCAGTGGTCATC3350.10293597996589286No Hit
GGCCCTTTCACTTGGGTTCTGTAACCCTCTCTGGTGTTGTGGTGGCTGAG3300.10139962205595417No Hit
GTAAATCTCAAAAAGGCTAGTATCGCCAAAACCATTCTGATGGGTCCATG3280.10078507889197867No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGT203.8636872E-4108.750011
CGTCTGT350.003564342662.1428571
CGCGGGG753.7532664E-758.0000041
TGTGAGT3000.058.0000042
GTGAGTC3250.055.769233
GTGGGCG400.006047231654.3750041
GCCGGCC400.006047231654.3750041
CGCTGGT400.006047231654.3750041
CCTGTAT450.00963333948.3333321
ACCGGCC1402.7102942E-1046.6071436
TGCGACA7400.045.067578
CAGACTG7400.044.087843
ACTGCGA7650.043.5947766
CACACCG1351.02227204E-842.9629633
TCAGACT7700.042.3701322
GCGACAG7750.042.096779
CGGCAAA1451.9210347E-840.02
CGGAGTA7300.039.72603145
CACCGGC1752.419256E-937.2857135
TGAGTCA5150.035.194174
Produced by FastQC (version 0.11.2)