FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_591.352000000a2c37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_591.352000000a2c37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18421
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG100.0070347968145.01
ATCCGGA100.0070347968145.02
TCGGAGA100.0070347968145.04
CAAGGTC100.0070347968145.09
CGGAGAG256.2964136E-6116.05
TCCGGAG203.8053707E-4108.753
GAGGGGC404.5565685E-9108.759
AAGGGAG451.1631014E-464.444453
AAGGGGC350.003511133462.1428579
GGAGAGG1102.1827873E-1159.318186
GAGAGGG1102.1827873E-1159.318187
TGGAGAG400.005957290554.3755
AGGGAGA701.6312746E-551.7857134
AGAGGGG1254.7348294E-946.3999988
GAAGGGA750.001445823638.6666642
GGGAGAG1051.784143E-434.5238085
GAGGGGG1450.001181278525.0000029
GGGGTTA300.001484005524.16666825-29
AAAAGGG703.3978722E-922.78571325-29
AAGGGGT604.4627996E-721.7525-29