FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_negative-2.351000000a2d53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_negative-2.351000000a2d53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5367
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT307157.22004844419602TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA1412.627166014533259TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGATGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT991.8446059250978202TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATATCGTAT851.5837525619526738TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTAT290.5403391093720886TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATTTCGTAT270.5030743432084964TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG200.3726476616359232TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCCCGTAT140.2608533631451463TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGCAT110.20495621389975777TruSeq Adapter, Index 1 (97% over 36bp)
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA70.13042668157257314No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTAT70.13042668157257314TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATATCGTA70.13042668157257314TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC70.13042668157257314TruSeq Adapter, Index 1 (96% over 33bp)
AGATCGGAAGAACACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA60.11179429849077697TruSeq Adapter, Index 13 (97% over 35bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA60.11179429849077697TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG60.11179429849077697Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATG100.006896311144.999983
CGGATGA100.006896311144.999984
GATGAGC100.006896311144.999986
ATCGGAT100.006896311144.999982
TGAGCAC100.006896311144.999988
GAGCACA4300.0118.0232549
AAGAGCA4250.0116.07
GAAGAGC4250.0116.06
AGAGCAC4250.0116.08
GGAAGAG4250.0116.05
GATCGGA4400.0115.3409041
CGGAAGA4350.0113.333344
ATCGGAA4350.0113.333342
TCGGAAG4450.0110.786523
AAAAATC200.00597078629.070-74
GCTTGAA3700.027.82432660-64
CTTGAAA3700.027.82432660-64
TATGCCG3850.027.49350545-49
TGCTTGA3700.027.43243460-64
CTTCTGC3700.027.43243455-59
Produced by FastQC (version 0.11.2)