FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_negative-1.351000000a2c2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_negative-1.351000000a2c2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7324
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT461162.95740032768978TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA2423.3042053522665213TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTAT1872.5532495903877663TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGATGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1431.9524849808847624TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATTTCGTAT580.7919169852539596TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG390.5324959038776624TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT380.5188421627525942TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGCAT230.3140360458765702TruSeq Adapter, Index 7 (97% over 36bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA190.2594210813762971TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCCCGTAT180.24576734025122884TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGCCTGAACTCCAGTCACCGGCTATGATCTCGTAT170.23211359912616056TruSeq Adapter, Index 2 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC170.23211359912616056TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACCGGCTATGATCTCGTAT140.19115237575095578TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTAT130.1774986346258875TruSeq Adapter, Index 7 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA100.1365374112506827TruSeq Adapter, Index 2 (97% over 35bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA90.12288367012561442TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACATCGTAT90.12288367012561442TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTTGTAT80.10922992900054614TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAACACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA80.10922992900054614TruSeq Adapter, Index 2 (97% over 35bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA80.10922992900054614TruSeq Adapter, Index 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATG100.006948329145.03
CGGATGA100.006948329145.04
ATCGGAT100.006948329145.02
TGAGCAC100.006948329145.08
AAGAGCA6150.0128.495947
GAAGAGC6150.0128.495946
CGGAAGA6150.0128.495944
GGAAGAG6150.0128.495945
GAGCACA6350.0127.8740169
AGAGCAC6250.0127.68
GATCGGA6400.0126.8751
TCGGAAG6250.0126.443
ATCGGAA6250.0126.442
AGATCGG750.001388908538.6666681
GATATCG304.285144E-529.040-44
TGATATC304.285144E-529.040-44
GTCTTTT200.005999121329.050-54
TTGCTTG200.005999121329.055-59
TTGAAAA5850.028.25640960-64
CTTGAAA5800.028.2560-64
Produced by FastQC (version 0.11.2)