FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_mr755100000-1.351000000a2c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_mr755100000-1.351000000a2c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19343
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1117357.76249806131417TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTAT4242.1920074445535853TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGATGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT3651.8869875407124024TruSeq Adapter, Index 2 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA1670.8633614227369074TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATTTCGTAT1410.7289458718916404TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT970.5014734012304193TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG660.3412087059918317TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGCAT560.2895104172051905TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCCCGTAT470.24298195729721347TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTAT390.20162332626790053TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACCGGCTATGATCTCGTAT350.18094401075324407TruSeq Adapter, Index 2 (97% over 35bp)
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA330.17060435299591584No Hit
GATCGGAAGAGCACACGCCTGAACTCCAGTCACCGGCTATGATCTCGTAT310.1602646952385876TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATGTCGTAT250.1292457219666029TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA240.12407589308793879TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGATGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTAT240.12407589308793879TruSeq Adapter, Index 2 (97% over 35bp)
TGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAGGACGCG240.12407589308793879No Hit
ATCCATTCTCCCTTTCCATGGATTGACCAGGTGGTTCTCCCAGTTGGTAC220.11373623533061056No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTATGATCTCGTAT210.10856640645194646TruSeq Adapter, Index 10 (97% over 38bp)
CGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCA210.10856640645194646No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC210.10856640645194646No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG100.0070374557145.01
CGGATGA550.0131.818184
ATCGGAT550.0131.818182
GATCGGA16350.0128.593261
TCGGAAG15900.0126.7613
GAGCACA16350.0126.3761449
CGGAAGA15900.0126.305024
GAAGAGC15950.0125.909096
AGAGCAC15850.0125.788658
ATCGGAA16100.0125.636642
GGAAGAG16000.0125.5156255
AAGAGCA16150.0123.9009257
TGAGCAC600.0120.8333368
GGATGAG650.0111.538475
GATGAGC650.0111.538476
TCGGATG750.096.666673
ATGAGCA750.096.666677
TATCGTA950.029.040-44
CCATCAC200.00604766529.0135-139
GCTTGAA14750.028.70508460-64
Produced by FastQC (version 0.11.2)