FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_mr75510-2.351000000a2d39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_mr75510-2.351000000a2d39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7397580
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA370680.5010827865328932No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG183130.2475539297986639No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA162400.21953125211217722No Hit
CAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACA160800.21736838263323954No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG150290.20316103374346745No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA140740.19025140654105802No Hit
TGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTCC125320.16940675193779586No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG120540.16294517936946948No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC118160.15972791101954964No Hit
CAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTGTG111010.15006258803554676No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT102050.13795051895349558No Hit
CCTCAATCCACACTCTATTCCACACCATGAGCATGTCCTCAGTGGTCATC101890.13773423200560184No Hit
GTAAATCTCAAAAAGGCTAGTATCGCCAAAACCATTCTGATGGGTCCATG101890.13773423200560184No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA101620.1373692477810311No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT100310.13559839839515084No Hit
GGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC99780.13488194788025273No Hit
CTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATA96040.12982624047323585No Hit
TGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGT95240.12874480573376698No Hit
CTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAG94610.12789317587643526No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA92570.1251355172907897No Hit
GGGAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGG91510.12370261626099346No Hit
GCCAAGGCAGACATTGAGATGGCTGGACCCATGGCTGCAGTAGGCTTGCT90280.12203991034906009No Hit
CGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCA89100.12044479410834354No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA86740.11725456162691042No Hit
GGGAGAACCACCTGGTCAATCCATGGAAAGGGAGAATGGATGACCACTGA85890.11610553721622477No Hit
GTTTGCTACCACATTGGGAGTGAACAAGTGCCACGTACAGATCATGGACC84790.11461856444945509No Hit
CAGAACTCTGGCGTTGGCCGTGATTAAATACACATACCAAAACAAAGTGG84140.11373989872363664No Hit
CCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCAGCAGGAGGCCAA84080.11365879111817649No Hit
GGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAAA83940.11346954003876944No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT82800.11192849553502632No Hit
CCTGGACATTCCTCAAACCGGATTTCAAGCTCTTCACTGTGCCATGGCCC81480.11014412821490271No Hit
GGCGAGGCGGCTGCCATTTTTATGACTGCCACACCACCAGGAACCCGTGA80330.10858956577691624No Hit
CGCAGATGACACTGCTGGCTGGGACACCCGCATTAGTAAGTTTGATCTGG79550.10753516690593411No Hit
GGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCATG79000.10679168052254928No Hit
CCGGTGTCTGCCCCAGCATGCCAAGGCAATGGGATGTCATGAAACCACTC78930.10669705498284575No Hit
GGCCCTTTCACTTGGGTTCTGTAACCCTCTCTGGTGTTGTGGTGGCTGAG78430.1060211582706777No Hit
CCGCTTTCCAGCCTTTGTCAGACAGGCTGCGATTTCATTTCCGTTTCTCA77990.10542636916396983No Hit
GGGAAAAGGACGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCA77780.10514249254485926No Hit
GTCGTTCTCCTCAATCCACACTCTATTCCACACCATGAGCATGTCCTCAG74590.10083027152122723No Hit
CTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAAACACAAC74330.10047880523089985No Hit
GCGGAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGG74130.10020844654603261No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA100.0070917713145.000026
CGACGAA100.0070917713145.000028
ACCGACG100.0070917713145.000026
CGTTATA100.0070917713145.000025
TACATAA400.0145.000024
CGCGACA1150.0126.086968
TTTCGAC351.904482E-9124.285728
CCCGTAC1100.0118.636371
CCGTACT256.467628E-6116.0000152
GATATTA458.54925E-11112.777784
CCCGTAA404.820322E-9108.750017
TATCTTA404.820322E-9108.750016
TATCTAA203.8670446E-4108.750018
CGTAGTA203.8670446E-4108.750013
TATAACA553.6379788E-12105.454556
TCGAAAT900.0104.722227
CTTATGT352.7635178E-7103.5714346
GTCGGTT352.7635178E-7103.5714341
AACGCTC501.9645086E-10101.500019
TTGCGAC501.9645086E-10101.500018
Produced by FastQC (version 0.11.2)