FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_mr75510-1.351000000a2c03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_mr75510-1.351000000a2c03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9205939
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA315060.34223559378353474No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA208120.22607145235266063No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG199590.21680569467166794No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA186770.20287990176776102No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC179980.19550422830305522No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA174640.18970362501858853No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG169540.18416372300533385No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG161300.175212979360389No Hit
CTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATA149350.16223222856462552No Hit
CTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAG143180.15553003338388405No Hit
GGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC137690.14956649180490986No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA124540.13528223465308645No Hit
CAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACA124300.13502153338187448No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT121490.1319691559981008No Hit
GGCTCATGTGGCTTTCGGAAATTGAACCAGCCAGAATTGCATGTGTCCTC119910.13025287262928856No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT119490.12979664540466757No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA117580.12772189778793885No Hit
CAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTGTG117400.12752637183452986No Hit
GGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTT112040.12170404344412884No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT111280.12087848941862422No Hit
GTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAGGACGC109660.11911875583794332No Hit
GCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTTC107580.11685934482077276No Hit
CCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCATC106760.11596861547746515No Hit
GGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCATG105790.11491494783965003No Hit
GGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAAA103770.1127207121402825No Hit
GCGGAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGG103760.11270984958731534No Hit
TGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTCC103480.11240569810423467No Hit
GGTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGAC102730.11159100663169721No Hit
GTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGGCAACGACTGT101810.11059165175871792No Hit
GGCAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTG101710.11048302622904628No Hit
GGCGAGGCGGCTGCCATTTTTATGACTGCCACACCACCAGGAACCCGTGA101400.11014628708706413No Hit
GGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCC100450.10911434455518335No Hit
CGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCA100110.1087450177542997No Hit
GCGGGAGTGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCC99990.1086146671186937No Hit
GGAGCATCCGGGAGACTGCCTGTCTTGCAAAATCATATGCGCAGATGTGG99870.1084843164830877No Hit
CCGGTGTCTGCCCCAGCATGCCAAGGCAATGGGATGTCATGAAACCACTC97270.10566005271162454No Hit
GCCAAGGCAGACATTGAGATGGCTGGACCCATGGCTGCAGTAGGCTTGCT96020.10430223359072877No Hit
CAGAACTCTGGCGTTGGCCGTGATTAAATACACATACCAAAACAAAGTGG95890.10416102040215561No Hit
GGCCAGTGCATGGCTCATGTGGCTTTCGGAAATTGAACCAGCCAGAATTG95720.10397635700171379No Hit
CTGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTC94570.10272716341048968No Hit
GTCAGGTGGGACCTGGGTTGATGTTGTCTTGGAACATGGAGGCTGCGTTA93750.10183643406718207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA100.0070918145.08
CCTAACG306.511982E-10145.06
CGTAATG100.0070918145.09
TACCGAC256.467715E-6116.08
CATTCGG458.54925E-11112.777775
TCGAAAT850.0110.882357
CGCGACA1000.0108.758
ATTCAGT600.0108.756
CGATAAT203.8670757E-4108.758
CGACAGG750.0106.3333361
TACAACG1000.0101.56
GTGTCGT800.099.68754
TAAGCCG800.099.68758
GTAAAAT800.099.68758
GTCTCGA1250.098.6000064
ACGTTAA750.096.666674
CTTAAGT451.0926669E-896.6666647
TAAAAGC451.0926669E-896.6666647
TACATAA451.0926669E-896.6666644
CTAGTAC607.2759576E-1296.6666647
Produced by FastQC (version 0.11.2)