FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_672.351000000a2d2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_672.351000000a2d2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT883754.77592512241989TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA4182.5909626231946943TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGATGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT2871.7789623752556871TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATATCGTAT1410.8739849996900763TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGCAT740.45868716295791234TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG730.4524886877828055TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTAT700.4338932622574847TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATTTCGTAT670.4152978367321639TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCCCGTAT480.29752680840513235TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACTCTCGCGCATCTCGTAT400.24793900700427696TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAACTCGTAT310.19215273042831463TruSeq Adapter, Index 8 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCC270.16735882972788693TruSeq Adapter, Index 8 (96% over 33bp)
AGATCGGTAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA270.16735882972788693Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAAGAACACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA220.13636645385235233Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA220.13636645385235233TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCACGTAT200.12396950350213848TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTA100.007026653145.0145
CGGATGA400.0145.04
TTCCTAC100.007026653145.0145
TCGGATG450.0128.888893
ATCGGAT450.0128.888892
TGAGCAC450.0128.888898
GATCGGA12850.0118.482481
ATCGGAA12450.0117.630522
GGAAGAG12500.0117.165
TCGGAAG12550.0116.693223
GAAGAGC12500.0116.586
GAGCACA13000.0116.55779
GGATGAG501.8189894E-12116.05
CGGAAGA12650.0115.770754
AAGAGCA12600.0115.654767
AGAGCAC12600.0115.654768
GATGAGC553.6379788E-12105.454556
ATGAGCA702.7284841E-1182.857147
AGATCGG2451.6807462E-929.5918371
CATATCG200.00604178129.040-44
Produced by FastQC (version 0.11.2)