FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_671.351000000a2d1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_671.351000000a2d1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8320
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT450054.08653846153846TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA2563.076923076923077TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGATGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT1351.622596153846154TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATATCGTAT961.153846153846154TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG380.4567307692307693TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGCAT350.4206730769230769TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTAT290.34855769230769235TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATTTCGTAT270.3245192307692308TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACTCTCGCGCATCTCGTAT190.22836538461538464TruSeq Adapter, Index 3 (97% over 34bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA190.22836538461538464TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCCCGTAT160.19230769230769232TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAACTCGTAT160.19230769230769232TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAGCTCGTAT100.1201923076923077TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAACACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA100.1201923076923077Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCACGTAT90.10817307692307693TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATGTCGTAT90.10817307692307693TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATG151.1868604E-4145.000023
CGGATGA151.1868604E-4145.000024
TCGGAAG6450.0118.0232543
GAGCACA6450.0118.0232549
CGGAAGA6400.0117.81254
ATCGGAA6500.0117.115382
GATCGGA6700.0116.865671
AAGAGCA6400.0116.679697
GAAGAGC6400.0116.679696
AGAGCAC6400.0116.679698
GGAAGAG6500.0115.999995
GATGAGC203.7309364E-4108.756
ATCGGAT203.7309364E-4108.752
TGAGCAC203.7309364E-4108.758
ATGAGCA203.7309364E-4108.757
GGATGAG300.001868574972.500015
AAAATTC200.006008507729.070-74
GCTTGAA5550.028.4774860-64
GCCGTCT5550.028.4774850-54
TATGCCG5700.028.23684345-49
Produced by FastQC (version 0.11.2)