FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_662.351000000a2d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_662.351000000a2d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15539
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT363623.399189137010104TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA1440.9267005598815883TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATATCGTAT660.42473775661239466TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTAT320.20593345775146407TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATTTCGTAT300.19306261664199756TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG300.19306261664199756TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGCAT260.16732093442306453TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATG202.064433E-6145.03
CGGATGA256.2646504E-6116.04
ATCGGAT301.5500631E-596.6666642
GATGAGC353.3314238E-582.857146
TGAGCAC353.3314238E-582.857148
GAGCACA7700.079.090919
GATCGGA7950.078.4276661
AGAGCAC7550.076.821198
GGAAGAG7750.075.774195
ATCGGAA7850.075.732482
AAGAGCA7750.074.838717
TCGGAAG7950.074.779873
CGGAAGA7950.073.867924
GAAGAGC7950.072.955976
GGATGAG406.458572E-572.55
ATGAGCA406.458572E-572.57
CAAATTC350.003500637462.1428573
CTTGAAA4450.027.04494360-64
TCTTCTG4250.026.95294255-59
GCCGTCT4600.026.47826250-54
Produced by FastQC (version 0.11.2)