FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_661.351000000a2cf4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_661.351000000a2cf4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369915
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT127233.4394387899922956TruSeq Adapter, Index 8 (97% over 36bp)
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA19630.5306624494816377No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG10890.29439195490856007No Hit
CAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACA8190.22140221402214022No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA6780.18328534933700985No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA6380.1724720543908736No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG6190.16733573929145887No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC6150.16625440979684522No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA6080.16436208318127138No Hit
TGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGT5600.15138612924590786No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA5480.14814214076206694No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA5130.13868050768419773TruSeq Adapter, Index 8 (97% over 36bp)
GGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC5010.13543651920035685No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG4540.12273089763864672No Hit
AGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCA4410.11921657678115243No Hit
GGAGCATCCGGGAGACTGCCTGTCTTGCAAAATCATATGCGCAGATGTGG4400.11894624440749901No Hit
CCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCAGCAGGAGGCCAA4360.1178649149128854No Hit
CTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAG4300.11624292067096496No Hit
CTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATA4270.11543192355000471No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA4230.1143505940553911No Hit
GAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATC4160.11245826743981725No Hit
GCTGTTTTGCGTTTTCCGGGGGGTCTCCTCTAACCACTAGTCCCTCTGCT4040.10921427895597637No Hit
GTAAATCTCAAAAAGGCTAGTATCGCCAAAACCATTCTGATGGGTCCATG4000.10813294946136275No Hit
GCAGGATGGAGCATCCGGGAGACTGCCTGTCTTGCAAAATCATATGCGCA3930.1062406228457889No Hit
GGCGAGGCGGCTGCCATTTTTATGACTGCCACACCACCAGGAACCCGTGA3900.10542962572482868No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT3900.10542962572482868No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT3890.10515929335117527No Hit
GGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCC3860.10434829623021506No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT3720.10056364299906735No Hit
GCGGGAGTGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCC3710.10029331062541395No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATG100.0070890696145.07
AACTTCG100.0070890696145.05
ACTTCGA100.0070890696145.06
GCCGCGT151.2293839E-4145.01
CGGGCGG202.1288142E-6145.01
GAAACCG100.0070890696145.06
CGGGCAT151.2293839E-4145.01
GCGTGTA100.0070890696145.02
GGATTGG100.0070890696145.04
TATAACT100.0070890696145.08
CGGGGCC203.864111E-4108.751
GGCCTCT259.38204E-487.01
CGGACCT259.38204E-487.03
CACACCG950.083.947373
CCCCGGT300.00193477572.51
ACCGGCC1300.066.923076
CGGGGAG651.3956196E-766.923071
CACCGGC1450.060.05
CGGCCGC1500.058.08
CCGGCCG1550.056.1290327
Produced by FastQC (version 0.11.2)