FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_652.351000000a2ce7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_652.351000000a2ce7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17858
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT493827.651472729308995TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA3111.7415164072124538TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATATCGTAT1090.6103707022062941TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATTTCGTAT420.2351887109418748TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG380.21278978609026766TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTAT340.19039086123866053TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGCAT200.11199462425803561TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGT100.0070330394145.000028
GCGGTAC100.0070330394145.000021
TGGGGGT100.0070330394145.000029
AAGAGCA9500.068.684217
GAGCACA9550.068.324619
AGAGCAC9600.067.968758
GAAGAGC9750.066.923086
GGAAGAG10150.064.285715
TCGGAAG10300.063.3495183
CGGAAGA10450.062.4401934
ATCGGAA10550.061.8483472
GATCGGA10600.061.5566021
GCTTGAA4900.026.33673560-64
CTTGAAA5000.025.8160-64
TTGAAAA5200.025.37560-64
TGCTTGA5050.024.98019860-64
CTTCTGC5250.024.58095255-59
TCTGCTT5250.024.58095255-59
GAAAAAA5250.024.30476265-69
CTGCTTG5150.024.21359355-59
Produced by FastQC (version 0.11.2)