FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_651.351000000a2cda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_651.351000000a2cda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22474
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT314613.998398148972147TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA1780.7920263415502359TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATATCGTAT420.1868826199163478TruSeq Adapter, Index 6 (97% over 36bp)
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA380.1690842751624099No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG290.12903799946604966TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATTTCGTAT260.11568924090059624TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTG100.0070450855145.01
GGACATT259.26682E-487.04
GCATCTC350.003521270862.142857145
AGAGCAC9600.059.6614578
GAGCACA9850.058.883259
GAAGAGC9800.057.7040826
AAGAGCA10000.056.557
GGAAGAG10000.056.555
TCGGAAG10150.056.4285743
GATCGGA10500.055.9285741
ATCGGAA10550.054.9763032
CGGAAGA10350.054.637684
TAGAGTG450.00951778848.3333327
AACATAC200.006051820229.075-79
TCTGCTT4650.026.19354855-59
GCTTGAA4900.025.74489860-64
TGCTTGA4750.025.0315860-64
CCGTCTT5000.024.93999950-54
GCCGTCT5000.024.93999950-54
CTGCTTG4650.024.63440955-59
Produced by FastQC (version 0.11.2)