FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_642.351000000a2ccd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_642.351000000a2ccd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26425
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT9883.7388836329233683TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA410.15515610217596973TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG151.2165896E-4145.06
GAGCAAA100.007052084145.04
CACGCAA400.00598609354.3757
GAGCACA9150.026.939899
AGAGCAC9350.026.3636368
GAAGAGC9600.025.6770846
AAGAGCA9650.025.5440437
CGGAAGA9850.025.025384
TCGGAAG9900.024.8989893
GGAAGAG10050.024.5273635
ATCGGAA10050.024.5273632
GATCGGA10250.024.0487791
TTGAAAA2650.020.79245460-64
CTTGAAA2600.020.63461560-64
TGCTTGA2550.020.47058960-64
GCTTGAA2700.020.40740860-64
TGAAAAA2800.018.64285760-64
CTGCTTG2600.018.40384755-59
GAAAAAA2900.018.00000265-69
TCTGCTT2900.017.50000255-59
Produced by FastQC (version 0.11.2)