FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_641.351000000a2cb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_641.351000000a2cb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17787
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT428524.09062798673188TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA2101.1806375442739079TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGATGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1340.7533591949176365TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATATCGTAT620.3485691797380109TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATTTCGTAT290.16304042278068254TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG290.16304042278068254TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGCAT280.15741833923652104TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAACCTCGTAT210.11806375442739078TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCCCGTAT180.10119750379490638TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAG203.8031186E-4108.749995
TCGGATG203.8031186E-4108.749993
GATGAGC203.8031186E-4108.749996
ATCGGAT203.8031186E-4108.749992
CGGATGA259.2344906E-487.04
TGAGCAC259.2344906E-487.08
GAGCACA10150.075.714289
AGAGCAC10250.072.853658
ATGAGCA300.00190445972.57
GATCGGA10900.071.169721
AAGAGCA10500.071.119057
GAAGAGC10600.070.448116
TCGGAAG10800.069.814823
ATCGGAA10800.069.814822
CGGAAGA10750.069.465124
GGAAGAG10850.068.824885
GCTTGAA6250.024.82400160-64
TGCTTGA6050.024.6859560-64
CTTGAAA6300.024.62698460-64
CTTCTGC6150.024.52032555-59
Produced by FastQC (version 0.11.2)