FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_632.351000000a2ca4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_632.351000000a2ca4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12888
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT2682.079453755431409TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA200.15518311607697083TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGT100.0070101894145.02
CATTACC100.0070101894145.05
GCACATA100.0070101894145.07
GGGCACC100.0070101894145.03
GCTGGGA350.003486931862.1428575
ATAGATC2205.4801843E-419.772726145
CGTATGC1500.0024148668.700001140-144
GATCTCG1750.0096689177.457143135-139
AAAAAAA6452.0340667E-75.39534965-69
Produced by FastQC (version 0.11.2)