FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_631.351000000a2c97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_631.351000000a2c97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73823
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT5130.6949053817915826TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA100.007077655145.04
CGACGGA259.3520887E-487.08
TGATTAG300.001928621172.58
TTGATTA400.006028789654.3757
GAGAGGG1000.00603856329.06
GCCGTCT3302.4612036E-517.575758145
ATGCCGT3802.1100277E-1013.355262145
TCTCGTA4951.07320375E-1011.717172145
TATGCCG4050.010.740741145
CTTGAAA2100.006404204810.357142145
TGCCGTC3552.4946748E-610.211267145
TCACTCC10250.09.902439145
TCCGCGA8107.028575E-99.845679145
CGTATGC4350.09.666667140-144
CTCCAGT13700.09.525547145
GTATGCC4600.09.141304140-144
CCATTTT1300.00753662048.923077100-104
ACTCCGC9100.08.763737145
AATCTCG6003.6379788E-128.458334145
ATCTCGT5150.08.446602135-139
Produced by FastQC (version 0.11.2)