FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_622.351000000a2c8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_622.351000000a2c8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14660
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT4493.062755798090041TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA230.15688949522510232TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCAT100.0070202085145.04
CAAAGCT100.0070202085145.03
AGAAATG400.00593302554.3753
GAGCACA3503.6379788E-1226.928579
AGAGCAC3503.6379788E-1226.928578
AAGAGCA3605.456968E-1226.1805557
GAAGAGC3605.456968E-1226.1805556
GGAAGAG3759.094947E-1225.1333355
CGGAAGA3801.0913936E-1124.802634
GATCGGA4002.0008883E-1123.56251
TCGGAAG4002.0008883E-1123.56253
ATCGGAA4002.0008883E-1123.56252
CGTATGC1054.7293724E-1119.33333245-49
TCTGCTT1109.094947E-1118.45454455-59
TCGTATG1109.094947E-1118.45454445-49
CTTGAAA1109.094947E-1118.45454460-64
GTATGCC1059.858923E-1017.95238145-49
TGCCGTC1059.858923E-1017.95238150-54
TATGCCG1059.858923E-1017.95238145-49
CCGTCTT1059.858923E-1017.95238150-54
Produced by FastQC (version 0.11.2)