FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_621.351000000a2c7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_621.351000000a2c7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11078
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT2011.8144069326593248TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG100.006996915145.06
GATCTCT100.006996915145.01
AAGAGCA2353.3007607E-521.5957457
AGAGCAC2353.3007607E-521.5957458
GAGCACA2403.883301E-521.1458349
TCGGAAG2454.552811E-520.7142853
CGGAAGA2454.552811E-520.7142854
GGAAGAG2454.552811E-520.7142855
CTTGAAA704.0482246E-520.714285145
GAAGAGC2505.3199954E-520.36
ATCGGAA2556.19659E-519.9019622
GATCGGA2658.330655E-519.1509441
ATCTCGT602.3653435E-416.91666640-44
TGCCGTC602.3653435E-416.91666650-54
TCTCGTA602.3653435E-416.91666640-44
ATGCCGT602.3653435E-416.91666645-49
CTCGTAT602.3653435E-416.91666640-44
CCGTCTT550.002593075215.81818250-54
TTGAAAA550.002593075215.81818260-64
GCCGTCT550.002593075215.81818250-54
Produced by FastQC (version 0.11.2)