FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_612.351000000a2c60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_612.351000000a2c60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35554
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT13213.715475052033526TruSeq Adapter, Index 6 (97% over 36bp)
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAA1060.2981380435394049No Hit
GAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTT910.2559486977555268No Hit
GCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGG900.25313607470326827No Hit
GCGGGTTAGAGGGTTCATAACACAAGGGTAGTATCCCACCAGCGCCTCCT810.22782246723294144No Hit
CTCGGCTTAGGTCCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAA780.21938459807616584No Hit
GCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAG740.20813410586713169No Hit
GGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCG720.2025088597626146No Hit
CCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCA600.1687573831355122No Hit
CTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGTC590.16594476008325365No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACA560.15750689092647804No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACACCGAT540.15188164482196095No Hit
GTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAA500.1406311526129268No Hit
CCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCAT480.13500590650840974No Hit
CAAACACTCAATATCAAACTACAGTAAAGCTCCATGGGGTCTTTCCGTCC480.13500590650840974No Hit
GTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATGA470.1321932834561512No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCAACTTAACCTCGCATGCAATCG460.12938066040389268No Hit
CCTCGACCGATTAGTATTGGTCCGCTCCAACTATCACTAGCCTTCCACTT430.12094279124711706No Hit
GGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAA420.11813016819485853No Hit
CGTCCCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTG420.11813016819485853No Hit
GGGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTA420.11813016819485853No Hit
GAAAATTCGATTCCCTGAGTAGCGGCGAGCGAAACGGGAAGAGCCCAAAC420.11813016819485853No Hit
CGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGC410.11531754514259998No Hit
GTAGGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGA410.11531754514259998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA390.10969229903808292TruSeq Adapter, Index 6 (97% over 36bp)
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTATGACT380.10687967598582437No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACAC380.10687967598582437No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCGACTTAACCTCGCATGCAATCG380.10687967598582437No Hit
GGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATG360.1012544298813073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATG202.102166E-6145.000023
ATCGGAT256.378821E-6116.02
CTTTAGT203.8351232E-4108.750011
CGGATGA301.5782301E-596.6666644
ACCGATC259.3119196E-487.04
ATACTCC259.3119196E-487.06
TGAGCAC353.391785E-582.857148
GATGAGC406.575254E-572.500016
AAATACT300.001920367872.54
CCGATCC300.001920367872.55
CTCCCTA300.001920367872.59
CGCACGG300.001920367872.53
CGATCCC300.001920367872.56
AATACTC300.001920367872.55
ATGAGCA451.17814896E-464.444447
GCGGGTT350.003538281262.1428571
GCTAAAT350.003538281262.1428571
GGATGAG501.9838684E-458.05
CGACCGA501.9838684E-458.02
CTTCGGT400.00600318154.3750046
Produced by FastQC (version 0.11.2)