000000000-B4Y9M_l01n01_611.351000000a2c54.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-B4Y9M_l01n01_611.351000000a2c54.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83005
Sequences flagged as poor quality	0
Sequence length	151
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	4683	5.6418288054936445	TruSeq Adapter, Index 7 (97% over 36bp)
GAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTT	294	0.35419553038973556	No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAA	264	0.3180531293295585	No Hit
GCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAG	213	0.2566110475272574	No Hit
GCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGG	211	0.2542015541232456	No Hit
GGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCG	200	0.24094934040118066	No Hit
GCGGGTTAGAGGGTTCATAACACAAGGGTAGTATCCCACCAGCGCCTCCT	192	0.23131136678513345	No Hit
CTCGGCTTAGGTCCCGACTAACCCTGGGCGGACGAGCCTTCCCCAGGAAA	186	0.22408288657309802	No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACA	179	0.2156496596590567	No Hit
CCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCA	152	0.1831214987048973	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTAT	152	0.1831214987048973	TruSeq Adapter, Index 7 (97% over 36bp)
CTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGTC	149	0.1795072585988796	No Hit
CGTCCCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTG	132	0.15902656466477924	No Hit
CAAACACTCAATATCAAACTACAGTAAAGCTCCATGGGGTCTTTCCGTCC	129	0.15541232455876153	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA	129	0.15541232455876153	TruSeq Adapter, Index 7 (97% over 36bp)
GTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGTATGA	128	0.1542075778567556	No Hit
CGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGC	124	0.149388591048732	No Hit
CACGGTTTCAGGATCTATTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT	119	0.14336485753870248	No Hit
CCTCGACCGATTAGTATTGGTCCGCTCCAACTATCACTAGCCTTCCACTT	119	0.14336485753870248	No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACACCGAT	116	0.1397506174326848	No Hit
GTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAA	114	0.13734112402867296	No Hit
CCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGCAT	113	0.13613637732666709	No Hit
GGGCGTTGAAGGTCGATCGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAA	109	0.13131739051864347	No Hit
CACGAAAGGCGTAACGATTTGGGCACTGTCTCAACGAGAGACTCGGTGAA	105	0.12649840371061982	No Hit
GCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTAACAC	104	0.12529365700861395	No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCGACTTAACCTCGCATGCAATCG	104	0.12529365700861395	No Hit
GAAAGGCGTAACGATTTGGGCACTGTCTCAACGAGAGACTCGGTGAAATT	104	0.12529365700861395	No Hit
CCTCCATTGCTCAAACAAAAACAACTGGTACAGGAATATCAACCTGTTGT	102	0.12288416360460212	No Hit
CTCGCTTTCGCTGCGGCTCCGTCTCTTCAACTTAACCTCGCATGCAATCG	101	0.12167941690259623	No Hit
GGGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACTA	98	0.11806517679657852	No Hit
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTATGACT	97	0.11686043009457261	No Hit
GTACAAACACTCAATATCAAACTACAGTAAAGCTCCATGGGGTCTTTCCG	97	0.11686043009457261	No Hit
GGCCTATTCACTGCGGCTGACCGAAGTCAGCACCCCTTCTCCCGAAGTTA	94	0.1132461899885549	No Hit
GTAGGAGAGCGTTCTAAGGGCGTTGAAGGTCGATCGTGAGGACGGCTGGA	90	0.1084272031805313	No Hit
GAAAATTCGATTCCCTGAGTAGCGGCGAGCGAAACGGGAAGAGCCCAAAC	89	0.10722245647852538	No Hit
CCCCCATTCGGAAATCTCTGGATCATAGCTTACTTACAGCTCCCCAAAGC	88	0.10601770977651949	No Hit
CCCGTCACAACTTGTCCTTAAAGAGTAAAGCATTTAACTCTACTCAAGAC	87	0.10481296307451358	No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC	86	0.10360821637250767	No Hit
GGAAATTTGAGAGGAGCTGTCCTTAGTACGAGAGGACCGGGATGGACTTA	86	0.10360821637250767	No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG	84	0.10119872296849587	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATGA	20	3.8534412E-4	108.75001	4
ATCGGAT	20	3.8534412E-4	108.75001	2
TGAGCAC	30	1.5907906E-5	96.666664	8
GGATGAG	35	3.4187004E-5	82.85714	5
GATGAGC	45	1.2229157E-6	80.55556	6
ATGAGCA	40	6.62728E-5	72.50001	7
TCGGATG	30	0.0019294727	72.5	3
GCGGGTT	90	2.764864E-10	64.44444	1
AAACAGC	35	0.0035549945	62.142857	4
TCGTAAG	35	0.0035549945	62.142857	7
GGGCGCA	90	2.0190782E-8	56.38889	1
AGAAACT	40	0.0060314313	54.375004	3
CTAAATT	40	0.0060314313	54.375004	2
AGTTCGT	40	0.0060314313	54.375004	145
GGTGGTC	75	2.530247E-5	48.333336	145
AGAGCGT	45	0.009608261	48.333332	3
CAGCTCG	65	7.297617E-4	44.615387	3
TAGAGGG	115	1.391727E-7	44.130432	7
CCCGGTA	85	5.299109E-5	42.64706	2
GAGCGTT	70	0.0010510455	41.42857	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content