FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_592.351000000a2c47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_592.351000000a2c47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325445
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA18700.5745978583170736No Hit
CAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACA8290.25472814146783634No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG7420.227995513834903No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA6260.19235201032432517No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG5790.17791024597090138No Hit
CCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCAGCAGGAGGCCAA5350.16439029636344082No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT5030.15455760573983315TruSeq Adapter, Index 7 (97% over 36bp)
TGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGT5010.1539430625758577No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA4840.1487194456820661No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC4800.14749035935411514No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG4540.1395012982224339No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA4450.13673585398454424No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA4330.13304859500069136No Hit
GGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC4110.1262886201969611No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT4020.12352317595907142No Hit
GTAAATCTCAAAAAGGCTAGTATCGCCAAAACCATTCTGATGGGTCCATG3980.12229408963112046No Hit
TGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTCC3940.1210650033031695No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT3890.1195286453932308No Hit
CTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAG3740.11491957166341472No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA3720.11430502849943923No Hit
CCTCAATCCACACTCTATTCCACACCATGAGCATGTCCTCAGTGGTCATC3710.1139977569174515No Hit
CAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTGTG3650.11215412742552504No Hit
AGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCA3620.11123231267956184No Hit
CTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATA3610.11092504109757409No Hit
GCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTA3480.10693051053173347No Hit
CCTGGACATTCCTCAAACCGGATTTCAAGCTCTTCACTGTGCCATGGCCC3470.10662323894974574No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT3470.10662323894974574No Hit
GCTGTTTTGCGTTTTCCGGGGGGTCTCCTCTAACCACTAGTCCCTCTGCT3340.10262870838390513No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCA100.007088679145.000024
GCCGGTC100.007088679145.000023
CGTCTGT100.007088679145.000021
TTGCGAC100.007088679145.000028
CCGACCT100.007088679145.000023
GGGATGC203.8636872E-4108.750017
AGGGATG259.3810156E-487.06
CGGTGCT353.4337863E-582.857141
CGTGGTC300.001934565172.51
TCTCTTA300.001934565172.57
GGCCGCT350.003564342662.1428571
GCGCCGG350.003564342662.1428571
TAGCACT604.9401325E-448.3333329
GCTCTCC450.00963333948.3333325
CGCTCTC450.00963333948.3333324
GCCCGCT450.00963333948.3333321
CCCGCTC450.00963333948.3333322
AGGCCTA657.32926E-444.6153874
GCGGGCC657.32926E-444.6153871
GCTATAC2000.043.5145
Produced by FastQC (version 0.11.2)