FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n01_591.351000000a2c3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n01_591.351000000a2c3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18421
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT235312.77346506704305TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTAT1070.5808588024537213TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA590.32028662939036967TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACGC100.0070347968145.05
AAGTTAG100.0070347968145.06
GCAGGGT100.0070347968145.01
TTACCCC100.0070347968145.06
TTATTGA100.0070347968145.04
GAGAGAG259.239943E-487.03
GAGCACA6800.057.5735329
AGAGCAC6950.055.287778
TCGGAAG7250.053.03
CGGAAGA7300.052.636994
GAAGAGC7300.052.636996
GATCGGA7500.052.1999971
AAGAGCA7400.051.925687
ATCGGAA7450.051.577182
GGAAGAG7550.050.894045
CTTGAAA2950.027.52542560-64
TTGAAAA2950.027.52542560-64
TGCTTGA3000.027.06666860-64
CCGTCTT2900.027.00000250-54
CGTCTTC2700.026.85185250-54
Produced by FastQC (version 0.11.2)