FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_pr8.3520000009fe8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_pr8.3520000009fe8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131001
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG10030.7656430103586995No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA8860.6763307150327097No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT4600.35114235769192603No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT3660.27938718025053244No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3510.2679368859779696No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3260.248853062190365No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT3040.23205929725727284No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT2480.18931153197303838No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2350.17938794360348395No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC2300.17557117884596302No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2290.1748078258944588No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC2080.1587774139128709No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA2030.15496064915534996No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1990.15190723734933323No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1910.14580041373729974No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1850.1412202960282746No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1840.14045694307677042No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT1780.13587682536774529No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG1750.13358676651323273No Hit
GCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCAT1740.13282341356172853No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1660.12671658994969504No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1610.12289982519217411No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG1580.12060976633766154No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA1520.11602964862863642No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA1500.11450294272562805No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG1500.11450294272562805No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1490.11373958977412386No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1480.11297623682261967No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC1460.1114495309196113No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA1430.10915947206509874No Hit
GCATTATATGTCCAAATGTCCAGAAATCCATCATCAACTTTTTTATTTAA1420.10839611911359455No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1340.10228929550156104No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGG100.00708388145.06
GAGCGAA600.0132.916671
GCGCAAA203.8584793E-4108.751
GCAAAAG5900.089.703393
TTTTTAA259.368417E-487.01
TTTTAAA259.368417E-487.02
AGCAAAA6050.086.280992
GCAGGGG1950.078.076939
CAAAAGC7250.074.04
AAGCAGG8250.073.8181847
GCAGGGT2950.073.728829
AGGTAGT300.001931976472.56
TAATAGA406.641612E-572.51
TTTAAAC300.001931976472.53
TAAACTA300.001931976472.55
CAAGGTA300.001931976472.54
AACGCAG300.001931976472.56
GTAGTTT300.001931976472.58
GGTAGTT300.001931976472.57
AGCAGGG5200.071.1057748
Produced by FastQC (version 0.11.2)