FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_ny238_plasmid_mix.3520000009feb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_ny238_plasmid_mix.3520000009feb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149041
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG4410.2958917344891674No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT3060.20531263209452433No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT3020.20262880683838674No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG2670.17914533584718298No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT2600.17444864164894222No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA2600.17444864164894222No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT2540.17042290376473587No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG2460.16505525325246073No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA1920.12882361229460348No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG1910.1281526559805691No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT1850.12412691809636275No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT1780.119430223898122No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC1770.1187592675840876No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG1730.11607544232795004No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG1700.11406257338584685No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT1700.11406257338584685No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT1680.11272066075777806No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1670.11204970444374367No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG1550.10399822867533096No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC1540.10332727236129656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGC259.3709654E-487.05
TATAGGG259.3709654E-487.04
CGTCAGT259.3709654E-487.06
TCAGTCC259.3709654E-487.08
TTAATAC300.001932499772.53
TACTGAT1300.072.57
CAGTCCG300.001932499772.59
TTATAGG350.003560551762.1428573
TACTGCG350.003560551762.1428576
GACAGGC651.0891088E-555.7692347
ACAGGCC651.0891088E-555.7692348
ATGCGAG400.006040824654.3755
ATACTGA2200.049.431826
ATAGATC450.0096231748.3333326
CACCTCG604.931454E-448.3333321
GTCCAGA604.931454E-448.3333321
CTGGGAC901.3159388E-648.3333323
ATACATC901.3159388E-648.3333323
CATATAC2200.046.1363683
ACTGATG2400.045.31258
Produced by FastQC (version 0.11.2)