FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_extraction_neg_ctrl.3520000009fe9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_extraction_neg_ctrl.3520000009fe9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18319
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT860.46945793984387796No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC560.30569354222392053No Hit
GGTCTATGGTAGAAGGAAACGTATATAAGAAACTGAAATGAGGCCCATGT500.27294066269992906No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG490.26748184944593045No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT480.26202303619193185No Hit
CCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCC480.26202303619193185No Hit
TCCTAATCCAATTGTATTTTCACTTTCACCCCTACAAGATCCTCCTAATT460.25110540968393474No Hit
ATACAGGCCTACCTCAAATAAACAAAGAAAAGTCTCAAATATACTATCTA430.23472896992193898No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT400.21835253015994324No Hit
CTGCAGAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA390.21289371690594466No Hit
GGCCTGTATTGATACAAACTTCCCTCTTCAAACTGCCTTTGCTATGTCCT370.20197609039794748No Hit
GATAAGGGGCGTCTGTCAGGGCACCTCAGACCTCAAAGACGAGCTGAAGC350.19105846388995032No Hit
AAGCAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT350.19105846388995032No Hit
ACCCAGGCCTCCGATGCTCTTGGTTTTTTGATAGAGATTACATTGCATCT350.19105846388995032No Hit
CTTTAGGACATAGCAAAGGCAGTTTGAAGAGGGAAGTTTGTATCAATACA340.18559965063595174No Hit
AGCGAAAGCAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACAC340.18559965063595174No Hit
TCTGATAGGCCTGCAAATTTTCAAGAAGATCATTTTTCAGACCAGCACTG330.18014083738195316No Hit
ATTATAACCAAAGCATTATGGAACAAAAAATCATCTGCCAACAAACTGGA330.18014083738195316No Hit
GCCCTAGACTTTTGTTTGTTTGGAGGCTTTTGATTGCTGATTCCAGTTTC320.17468202412795458No Hit
AATCAGACATGAGAACAGAATGGTTTTAGCCAGCACTACAGCTAAGGCTA320.17468202412795458No Hit
TTTCTGGGCAGGCTGTTCTTGGGTGTGATCGAGAGGCTGGCTCAGGTGGC320.17468202412795458No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT310.169223210873956No Hit
CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT300.16376439761995742No Hit
ATAAAACACCAGTCGATAGAAATCAATACATGGAGACCAAATGAAAATTA300.16376439761995742No Hit
GCTTTGGGCTTTGTTTTTCTTTTTCCAATTCTTTCACATGTAAGGTTAGA300.16376439761995742No Hit
GCTTTGTCCATGTTATTTGGATCCCCGTTCCCATTAAGGGCATTTTGGAC300.16376439761995742No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT300.16376439761995742No Hit
ATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGTTCCTT300.16376439761995742No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCA300.16376439761995742No Hit
CTTCTCTGTTGTCCAGTTTGTTGGCAGATGATTTTTTGTTCCATAATGCT290.15830558436595885No Hit
GTGTTAAATGATGGGGCTTTCTGGGCAGGCTGTTCTTGGGTGTGATCGAG280.15284677111196027No Hit
GTTGTCCAGTTTGTTGGCAGATGATTTTTTGTTCCATAATGCTTTGGTTA280.15284677111196027No Hit
GTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG280.15284677111196027No Hit
TTATTATTCTTCCTACTAGCTTTGGGCTTTGTTTTTCTTTTTCCAATTCT280.15284677111196027No Hit
CCATAGACCTTTGTAAATTTTCCTCCCTTTCTCTGAAACATTTCCTGTTT280.15284677111196027No Hit
TGTCTAGCCTGACTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGA280.15284677111196027No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA280.15284677111196027No Hit
TTTAACACCTAGGCGCCCAGAAATGTTTTCAGAAAGATGCCTGCAACTCA270.14738795785796166No Hit
GCTAAAGGTTTATCAGTTTTATCTTTTCTAAGAACTGGCACTTGGGTTAA270.14738795785796166No Hit
GTCCTAGATTGGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATG260.1419291446039631No Hit
GAATAATAAAGATGAGTAGAAATAAAACAGAAACAAAAAGAAAAATTTAA260.1419291446039631No Hit
GTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAT260.1419291446039631No Hit
AAAGCAGGGGGAGCAGCAGGCAGAGGGAGAAGAGGGAGAAGCAGGCTCCC250.13647033134996453No Hit
AGATTACATTGCATCTGTAGATTGAAATCACCTGGAAAGCCACCTGGCCC250.13647033134996453No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA250.13647033134996453No Hit
TATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCA240.13101151809596592No Hit
CGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTA240.13101151809596592No Hit
GGCTAGACAAATGGTGCAAGCGATGAGAACCATTGGGACTCATCCTAGCT240.13101151809596592No Hit
TCCTAAAACATACAATCTTCCGAAACTGGAATCAGCAATCAAAAGCCTCC240.13101151809596592No Hit
GTAACAACCAACACCAAAGAAATCAAAGCATTATAACCAAAGCATTATGG240.13101151809596592No Hit
GCAGAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA230.12555270484196737No Hit
CCTTTAGCTATTCATCTAGAAAAAAGAAAGGACTCAAATAAATAAAATCA230.12555270484196737No Hit
GTCTGAGAAGGCTCAATGCGGTTATGCTTAACTGACATCTGAAGGATCTC230.12555270484196737No Hit
GTTCACAGGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCC230.12555270484196737No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA230.12555270484196737No Hit
GCATCTGGGAGGTCGCGGAGAAACATCTTGAGTTGCAGGCATCTTTCTGA230.12555270484196737No Hit
CTTCTAACCGAGGTCGAAACGTACGTACTCTCTATCATCCCGTCAGGCCC230.12555270484196737No Hit
ATTCTGTTCATCGTGATTCTGTTGGCTTTTCACGTCCTCAGTATTTCGTG220.12009389158796878No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG220.12009389158796878No Hit
CTCATGAGCGGATACATATTTGAATGTATTCTGCAGAAGAACATGTGAGC220.12009389158796878No Hit
AAAGCAGGCAGGTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCAGGAG220.12009389158796878No Hit
AAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGT220.12009389158796878No Hit
GGTTATAATGCTTTGATTTCTTTGGTGTTGGTTGTTACTTCTGCTTCATT220.12009389158796878No Hit
AGCGAAAGCAGGGGGAGCAGCAGGCAGAGGGAGAAGAGGGAGAAGCAGGC220.12009389158796878No Hit
GTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCAGGAGAAATTTAAGAA220.12009389158796878No Hit
GGTGTTAAATGATGGGGCTTTCTGGGCAGGCTGTTCTTGGGTGTGATCGA210.11463507833397019No Hit
ATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGTGATCCTCTCGC210.11463507833397019No Hit
ATCCTAATCCAATTGTATTTTCACTTTCACCCCTACAAGATCCTCCTAAT210.11463507833397019No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG210.11463507833397019No Hit
TTTCTGATAGGCCTGCAAATTTTCAAGAAGATCATTTTTCAGACCAGCAC210.11463507833397019No Hit
GGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTCTGCAGAAGA210.11463507833397019No Hit
GGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCACAATGTCAAGTGC210.11463507833397019No Hit
GAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG210.11463507833397019No Hit
GGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATA200.10917626507997162No Hit
ATCTAACCTTACATGTGAAAGAATTGGAAAAAGAAAAACAAAGCCCAAAG200.10917626507997162No Hit
GCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCAGCAGAGGC200.10917626507997162No Hit
TCTCGCTATTGCCGCAAATATCATTGGGATCTTGCACTTGACATTGTGGA200.10917626507997162No Hit
GCTTGGAATAGGGGGGCCAATTAGGAGGATCTTGTAGGGGTGAAAGTGAA200.10917626507997162No Hit
GTTCAAGTGATCCTCTCGCTATTGCCGCAAATATCATTGGGATCTTGCAC200.10917626507997162No Hit
ATAGCGAGAGGATCACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCT200.10917626507997162No Hit
GAAGTAACAACCAACACCAAAGAAATCAAAGCATTATAACCAAAGCATTA200.10917626507997162No Hit
ATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCAC200.10917626507997162No Hit
GAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAA200.10917626507997162No Hit
GTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATCAGAAA200.10917626507997162No Hit
GTTCTTGGGTGTGATCGAGAGGCTGGCTCAGGTGGCTTTCAGATGGACTC190.10371745182597303No Hit
CCCCTACAAGATCCTCCTAATTGGCCCCCCTATTCCAAGCACATGGGCCT190.10371745182597303No Hit
CCCAGAGTCCATCTGAAAGCCACCTGAGCCAGCCTCTCGATCACACCCAA190.10371745182597303No Hit
TCATTAAATAAAACACCAGTCGATAGAAATCAATACATGGAGACCAAATG190.10371745182597303No Hit
ATGTAATCTCTATCAAAAAACCAAGAGCATCGGAGGCCTGGGTGGCTCAA190.10371745182597303No Hit
GATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT190.10371745182597303No Hit
GTATATAAGAAACTGAAATGAGGCCCATGTGCTTGGAATAGGGGGGCCAA190.10371745182597303No Hit
GTCAGAGGGGAATGCCAGGTTTCTCTGACCCCAAAACTAGCCATTCCCCA190.10371745182597303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTG100.0070344843145.000027
AGGCAGG100.0070344843145.000025
CAGGTGT100.0070344843145.000028
TAGAAAC203.805034E-4108.750013
CAAGGTA259.239129E-487.09
ACAAGGT259.239129E-487.08
AACAAGG406.490085E-572.500017
AAACAAG350.003510826762.1428576
GAAACAA450.00948976448.3333325
AGTAGAA450.00948976448.3333321
GTTCTTG450.00948976448.3333321
TTCTTGG450.00948976448.3333322
CCTTTGT450.00948976448.3333328
TCTTGGG450.00948976448.3333323
ACCTTTG450.00948976448.3333327
CCATAGA450.00948976448.3333321
AGACCTT450.00948976448.3333325
GACCTTT450.00948976448.3333326
CTTTGTA450.00948976448.3333329
ACAACCA450.00948976448.3333325
Produced by FastQC (version 0.11.2)