000000000-B4Y2B_l01n02_3027_d1_nw.3520000009fb5a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-B4Y2B_l01n02_3027_d1_nw.3520000009fb5a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82016
Sequences flagged as poor quality	0
Sequence length	151
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA	642	0.7827740928599298	No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG	584	0.7120561841591885	No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG	319	0.38894849785407726	No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT	302	0.3682208349590324	No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT	250	0.30481857198595397	No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT	184	0.2243464689816621	No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG	170	0.2072766289504487	No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT	162	0.19752243464689817	No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT	154	0.18776824034334763	No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT	147	0.17923332032774095	No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC	144	0.1755754974639095	No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT	142	0.17313694888802184	No Hit
CTTTCCGTCTACTCTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCT	138	0.16825985173624658	No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC	130	0.15850565743269607	No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA	123	0.14997073741708936	No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC	121	0.14753218884120173	No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG	115	0.14021654311353882	No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA	115	0.14021654311353882	No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA	114	0.138997268825595	No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT	110	0.13412017167381973	No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC	109	0.13290089738587593	No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG	109	0.13290089738587593	No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC	107	0.1304623488099883	No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT	107	0.1304623488099883	No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG	104	0.12680452594615685	No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT	102	0.12436597737026922	No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT	102	0.12436597737026922	No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA	101	0.1231467030823254	No Hit
AGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT	100	0.12192742879438159	No Hit
GGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGT	100	0.12192742879438159	No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG	99	0.12070815450643778	No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC	98	0.11948888021849394	No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT	96	0.11705033164260631	No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT	95	0.1158310573546625	No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG	91	0.11095396020288724	No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC	90	0.10973468591494343	No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG	90	0.10973468591494343	No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA	90	0.10973468591494343	No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT	88	0.1072961373390558	No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT	88	0.1072961373390558	No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT	88	0.1072961373390558	No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA	87	0.10607686305111198	No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC	87	0.10607686305111198	No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT	87	0.10607686305111198	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTTT	10	0.0070790797	145.00002	9
GAGCGAA	45	0.0	145.0	1
GCAGGTA	75	0.0	116.00001	9
TCCACCT	25	6.429269E-6	116.0	1
CCTACTA	30	1.5906755E-5	96.666664	5
CACCTAC	30	1.5906755E-5	96.666664	3
CCACCTA	30	1.5906755E-5	96.666664	2
CGAAAGC	80	1.8189894E-12	81.56251	4
GCGAAAG	80	1.8189894E-12	81.56251	3
AGCGAAA	80	1.8189894E-12	81.56251	2
GCAGGGT	195	0.0	78.07693	9
TCTAGCT	40	6.626803E-5	72.50001	2
AGCTCTA	40	6.626803E-5	72.50001	5
ACTATAT	30	0.0019293894	72.5	4
CCACTAT	30	0.0019293894	72.5	2
GTAGAAA	50	2.2873865E-6	72.5	1
CGACTAA	70	2.7703209E-9	72.5	9
GACGACT	70	2.7703209E-9	72.5	7
ACGACTA	70	2.7703209E-9	72.5	8
GAGCAAA	340	0.0	68.23529	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content