FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3025_d-2_nw.3520000009fe4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3025_d-2_nw.3520000009fe4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166845
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGTCACTCTATATCTGCCCAAACCCATAGAACGTCCCACACCAAG2320.1390512151997363No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1830.10968263957565405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2550.0108.0392152
AGCGAAA2500.0107.2999951
CGAAAGC3050.090.327873
GAAAGCA5400.053.7037054
GTATATG1351.891749E-1048.3333361
GAGCAAA3700.047.0270231
GTATTAG700.001054108841.428571
CTACGAC700.001054108841.428573
GCAAAAG4250.040.9411743
ACTATAT907.455617E-540.277783
ATAGACC907.455617E-540.277783
CAAAAGC4500.038.6666644
AGCAAAA4600.037.8260882
AAAGCAG10350.037.1256035
TAGACCT800.002031762636.254
GTCACTC1655.965558E-835.1515167
TGGTGCC1251.2587923E-534.8145
ATATGTC1707.756353E-834.117653
TATGTCA1956.9176167E-933.461544
GTACATA900.003619636432.2222253
Produced by FastQC (version 0.11.2)