FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3024_d-2_nw.3520000009fc4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3024_d-2_nw.3520000009fc4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112961
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2630.2328237179203442No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1650.14606811200325776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA750.087.01
AGCGAAA1450.070.000011
TTATAGT350.003558320562.1428574
GCGAAAG1650.061.5151522
CGAAAGC1750.058.03
GAGCAAA3150.057.539681
CTAACCG400.00603705454.3754
GCAAAAG3450.050.434783
AGCAAAA3500.049.7142872
GCTAGGA604.9263483E-448.3333322
CTAGGGT450.00961718448.3333324
CAAAAGC4100.042.439034
GGGTAGC700.001052661641.428571
ATAGACC907.440304E-540.2777753
TCTAACC750.001477815938.6666643
CCAATAT800.002028989636.253
AGCAGGT2401.8189894E-1236.258
CTTCTAA1251.2552011E-534.81
GTACTAT1051.8433522E-434.5238081
GGTGCAC850.00273174934.1176451
Produced by FastQC (version 0.11.2)