FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3022_d-2_nw.3520000009f95c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3022_d-2_nw.3520000009f95c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160380
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2240.13966828781643595No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1970.12283327098141913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC100.0070853494145.000027
AGCGAAA2600.083.653851
GCGAAAG2900.075.02
CGAAAGC2950.073.728823
GAGCAAA5050.063.168321
GGAGCGA902.0350853E-856.388891
GAGCGAA1206.002665E-1154.3751
GCAAAAG6400.049.8437543
AGCAAAA6600.049.4318162
CAAAAGC7050.045.2482264
GTATAAG750.001479670438.6666681
AAAAGCA9000.035.4444435
GAAAGCA6400.035.117194
TATAGAC1251.2584886E-534.8000032
TAGATAC900.00361922332.222225
AAAGCAG12100.031.7561976
TAGGTAC1402.7324373E-531.071428145
GTATATA2859.094947E-1230.5263161
GTTGTGT1255.1347987E-429.0000028
CCTTAAC1000.006059462729.02
Produced by FastQC (version 0.11.2)