FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3021_d-2_nw.3520000009fdde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3021_d-2_nw.3520000009fdde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72768
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1170.1607849604221636No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATTG100.0070774495145.05
AGCGAAA702.910383E-1182.857141
GAGCAAA1950.078.076931
AGCAAAA2300.066.195652
GCAAAAG2300.066.195653
GCGAAAG954.456524E-1061.0526352
CAAAAGC2600.058.5576934
CGAAAGC1007.039489E-1058.03
GAGCGAA553.1991696E-452.7272721
AAAAGCA3350.045.447765
AAAGCAG4800.037.7604186
AGAAGTA800.002024248736.255
ATGCTGA800.002024248736.251
GAAAGCA2008.71114E-932.6254
AGAATGC1000.006038004529.08
AAGCAGG6300.028.7698427
GCAGGAC1358.0105197E-426.851853
GAGAATG1700.003065636621.3235287
AGCAGGT1800.00426943420.138898
TGATATG600.00469441414.50000155-59
Produced by FastQC (version 0.11.2)