FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3016_d8_nw.3520000009fd14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3016_d8_nw.3520000009fd14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18881
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC860.45548434934590326No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG610.3230761082569779No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT520.27540914146496476No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG450.23833483396006566No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA420.2224458450293946No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG410.2171495153858376No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT400.21185318574228057No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA380.20126052645516654No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG360.19066786716805253No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA320.16948254859382447No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA320.16948254859382447No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT300.15888988930671044No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT230.12181558180181133No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC200.10592659287114028No Hit
GAATTAAAAAATCTATACACTGAAAACTAGAAAACACTGATGAAAGAAAT190.10063026322758327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAT100.0070361574145.000022
CAGCTCG100.0070361574145.000029
ATTGATC100.0070361574145.000023
GAGCAAA404.5620254E-9108.750011
CGAAAGC352.657198E-7103.5714344
GCGAAAG352.657198E-7103.5714343
AGCGAAA301.5589008E-596.6666642
GAGCGAA405.8864316E-790.625011
CAAAAGC607.612107E-872.54
GCAAAAG607.612107E-872.53
ATCGAAA300.00190630972.52
AGCAAAA651.325152E-766.923072
AAAGCAG1300.066.923076
ATATACA350.00351247362.142864
AAGCAGA350.00351247362.142868
CTGCAGG350.00351247362.142864
TATAGAC350.00351247362.142862
AAAAGCA753.5658377E-758.0000045
TCGCACG400.00595955654.3750048
AGGCAGT400.00595955654.3750046