FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3013_d8_nw.3520000009fbfa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3013_d8_nw.3520000009fbfa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89998
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3920.4355652347829952No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA3580.3977866174803885No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2230.2477832840729794No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2080.23111624702771175No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1850.2055601235583013No Hit
GAGCAAAAGCAGGCATATCATCTACGAAGGAATGGCAGTGTTGGGGATTT1750.19444876552812285No Hit
GGGATGTACAGTGGGGGCCACTACTATGGAAAACCGCAGCTCCTCAAAAA1750.19444876552812285No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT1610.17889286428587303No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG1490.16555923464965888No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1450.1611146914375875No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1290.14333651858930196No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA1270.1411142469832663No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1140.12666948154403432No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG1060.11778039511989155No Hit
GAGCAAAAGCAGGGGATGCTGCAGGCAGAGAGAGAAGCAGGCTCCCCACT1040.11555812351385587No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA960.1066690370897131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCAC100.007080215145.02
GAGCAAA3650.0103.2876741
GTATGAC259.358801E-487.01
TATAGTA259.358801E-487.05
AGCAAAA4350.086.6666642
GCAAAAG5000.075.43
AGCGAAA1550.074.8387151
CGAAAGC1650.074.6969763
CAAAAGC5550.069.234244
GCGAAAG1800.068.472222
TCTACGC350.003555963762.142857145
GAGCGAA1200.060.4166681
AAAAGCA6250.060.3199965
CAATTGT553.2032523E-452.727276145
AAAGCAG8750.050.542866
ATATAGT604.920958E-448.3333324
AGCAGTA604.920958E-448.3333327
TATATAG657.3008955E-444.6153873
AGCAGGT2850.043.2456138
TAGAAAC1053.7853388E-641.428572
Produced by FastQC (version 0.11.2)