FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3011_d5_nw.3520000009fe20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3011_d5_nw.3520000009fe20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177124
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG24621.3899866760009938No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA20021.1302816106230664No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA15870.8959824755538492No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT11670.65886045933922No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT7390.41722183329193113No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC7200.40649488493936453No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG7050.39802624150312776No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT6400.36132878661276846No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG6160.3477789571147897No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC4730.26704455635599916No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4630.2613987940651747No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4560.25744676046159753No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4210.23768659244371176No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT4120.2326054063819697No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC4050.22865337277839254No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG4040.22808879654931008No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC3980.22470133917481538No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3900.22018472934215577No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT3690.20832862853142434No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC3590.2026828662405998No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA3360.18969761297170343No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3330.1880038842844561No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3130.17671235970280708No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG3120.17614778347372462No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG2980.1682437162665703No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG2890.16316253020482824No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG2750.15525846299767396No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT2710.15300015808134415No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA2690.15187100562317923No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2660.15017727693593189No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2590.14622524333235473No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA2520.14227320972877758No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG2490.14057948104153023No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA2280.12872338023079877No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC2280.12872338023079877No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC2240.12646507531446896No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT2220.12533592285630404No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC2220.12533592285630404No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC2210.1247713466272216No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC2190.1236421941690567No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT2150.12138388925272689No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC2130.12025473679456199No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2120.11969016056547954No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA2120.11969016056547954No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC2090.11799643187823221No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT2090.11799643187823221No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG2080.11743185564914976No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2050.1157381269619024No Hit
GGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGT2030.1146089745037375No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT2030.1146089745037375No Hit
AGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA1980.11178609335832523No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC1970.11122151712924279No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG1940.10952778844199543No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG1900.10726948352566562No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC1870.10557575483841827No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT1840.10388202615117094No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1810.10218829746392359No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG1790.10105914500575869No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1550.0130.967741
GAGCAAA12400.0123.3669361
TGATAGG203.8607477E-4108.750013
AGTAGAA1800.096.6666641
AGCAAAA16450.093.875382
GCAGGGT6700.093.05979
TAGATAC259.373907E-487.02
AAGCAGG20400.085.649517
GCAAAAG18050.084.7506943
AGCAGGG8650.083.815038
AGATCAG353.4294553E-582.857145
ATCAGTG353.4294553E-582.857147
CAGTGTG353.4294553E-582.857149
CAAAAGC18900.081.322754
AAAGCAG22250.078.528086
AAAAGCA19700.078.388325
AGCGAAA3350.077.9104461
TAGAAAC2250.077.333333
GTAGAAA2250.077.333332
CGAAAGC3450.075.6521763
Produced by FastQC (version 0.11.2)