FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3010_d8_nw.3520000009fa24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3010_d8_nw.3520000009fa24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151726
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG8440.5562659003730408No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG5090.33547315555672724No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA5010.33020049299394966No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT4560.3005417660783254No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT3490.2300199043011745No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3300.2174973307145776No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2620.17267969893096766No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2560.16872520200888444No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2360.15554354560194034No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT2220.14631638611707948No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA2180.14368005483569066No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1990.13115748124909377No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1890.1245666530456217No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1800.11863490766249687No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1540.10149875433346954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1650.0114.2424241
GAGCAAA5550.0112.342351
AGCAAAA6700.093.059712
ACCCGTC259.3712937E-487.07
AGCGAAA2250.077.3333362
GCGAAAG2300.075.6521763
GCAAAAG8500.075.058823
CGAAAGC2350.074.042564
AATACGA300.001932567372.56
GTAGAAA1350.064.444441
CAAAAGC10300.061.941754
GCAGGGT4100.061.8902449
AAAGCAG13750.060.109096
GATGTAC753.739824E-758.03
GATAGAC502.0047878E-458.02
AAGCAGG15000.056.5500037
AAAAGCA11550.056.4935075
TAGAAAC1850.054.864862
GACACGG400.006041035554.3757
TAAACGT400.006041035554.3754
Produced by FastQC (version 0.11.2)