FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3009_d8_nw.3520000009fa31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3009_d8_nw.3520000009fa31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168296
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10010.5947853781432714No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA6290.3737462565955222No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG6220.36958691828682794No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC3900.23173456291296288No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3420.20321338593905974No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3320.19727147406949658No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3090.18360507676950136No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2890.17172125303037505No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2620.15567809098255453No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2520.1497361791129914No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA2350.13963492893473403No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT2170.12893948756952037No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2170.12893948756952037No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT2140.12715691400865142No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2020.12002661976517565No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1920.11408470789561251No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1880.11170794314778723No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT1880.11170794314778723No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1870.11111375196083091No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1870.11111375196083091No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG1850.10992536958691829No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1820.10814279602604933No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1700.10101250178257357No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1690.10041831059561725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA5850.0111.538461
AGCAAAA6950.090.7553942
GCAAAAG8100.079.660493
AGCGAAA2300.069.3478241
CAAAAGC9650.066.865284
GAGCGAA1500.062.8333321
AAAAGCA10550.060.4739345
GCAGGTA2550.059.705889
CGAAAGC2750.058.03
GCGAAAG2750.058.02
ATCCCGA753.7422978E-758.06
CCGACCA753.7422978E-758.09
CAATTGT2800.056.964287145
AAAGCAG13500.054.7777756
AGCAGGT4750.050.368428
CAATTCG450.00962531648.3333329
TATCCCG901.3169629E-648.3333325
CCCGACC901.3169629E-648.3333328
AAGCAGG16400.045.5335357
GTATGCT1801.8189894E-1244.3055531
Produced by FastQC (version 0.11.2)