FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3007_d8_nw.3520000009fc3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3007_d8_nw.3520000009fc3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75725
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG5890.7778144602178937No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3620.47804555959062395No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG3020.3988114889402443No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC2620.3459887751733245No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1880.24826675470452295No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1730.22845823704192803No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1570.20732915153516013No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1390.18355893034004622No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1330.17563552327500825No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1330.17563552327500825No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1300.17167381974248927No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1230.16242984483327833No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1200.15846814130075934No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1170.15450643776824036No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1170.15450643776824036No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1060.1399801914823374No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1010.13337735226147243No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1000.13205678441729946No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC960.12677451304060747No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC950.12545394519643446No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA940.12413337735226147No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT920.12149224166391548No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG870.1148894024430505No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA860.11356883459887751No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG840.11092769891053152No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA820.10828656322218552No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG810.10696599537801256No Hit
GGGATGTACAGTGGGGGCCACTACTATGGAAAACCGCAGCTCCTCAAAAA810.10696599537801256No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC790.10432485968966657No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT760.10036315615714758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACGT100.007078013145.09
GTATCGA100.007078013145.08
GAGCAAA4000.0123.251
AGCAAAA4650.0106.021512
GCAGGTA1300.089.2307749
GCAAAAG5850.084.2735063
CGAAAGC801.8189894E-1281.56253
GAGCGAA554.339563E-879.090911
CAAAAGC6500.076.961544
AAAAGCA6750.074.111115
GCGAAAG903.6379788E-1272.52
ATAGACC607.931703E-872.53
AGCGAAA809.640644E-1172.51
AGTAGAA702.7666829E-972.51
AGCAGGT2800.069.910718
AAAGCAG7900.068.829126
AGACCTC651.3806857E-766.923085
CTCTAGC350.003553785462.1428571
GCAGGGT2350.061.702139
TATAGAC954.474714E-1061.0526352
Produced by FastQC (version 0.11.2)